HEMATOPOIETIC STEM CELLS
AND LEUKEMIA

     Our body produces millions of new blood cells each second. All of these blood cells derive from hematopoietic stem cells (HSCs). For proper functioning, HSC requires the specialized microenvironment – the niche. However, during aging the blood forming potential of this dynamic system declines. Additionally, HSCs accumulate mutations what may lead to leukemia.

     We study how HSCs contribute to blood production during aging and their role in hematopoietic malignancies. Our research focuses on HSCs' heterogeneity and mechanisms that regulate their aging. We trace the roots of leukemia within the pool of human HSCs. Finally, we want to find the cellular and molecular mechanism that governs the specific HSC-niche interactions.

     To answer these question we develop new mice models and cooperate with the leaders in the field.

>>> TEAM MEMBERS

>>> CURRENT PROJECTS

Group Leader
Krzysztof Szade 
krzysztof.szade[at]uj.edu.pl

PhD Students
Izabella Skulimowska
Monika Żukowska

Research Assistant
Kacper Kowalski 

Undergrad Students
Monika Gońka
Jan Morys
Sylwester Mosiołek 

Intern

Marek Piprek, MD

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  • Role of Neogenin-1/Netrin-1 axis
    in aging of hematopoietic stem cells
      ​

  • Contribution of pre-leukemic hematopoietic stem cells
    to acute lymphoid and myeloid leukemias

   

  • Heterogeneity and function
    of bone-marrow endothelial cells

   

  • Finding the determinants of human hematopoietic and leukemic stem cells niche.

>>> RECENT PAPERS

>>> NEWS

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  • "Neogenin-1 distinguishes between myeloid-biased and balanced Hoxb5+ mouse long-term hematopoietic stem cells"

  • "Heme oxygenase‐1 deficiency triggers exhaustion of hematopoietic stem cells" 

   

  •  "Cobalt protoporphyrin IX increases endogenous G‐CSF and mobilizes HSC
    and granulocytes to the blood"  

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>>> OUR ACHIEVEMENTS

>>> OUR METHODS

  • in cooperation with Irv Weissman laboratory, we demonstrated that Neogenin-1 marks the myeloid-biased fraction of strictly defined Hoxb5+ HSC in mice   ​

  • we showed that lack of extrinsic
    heme-oxygenase-1 triggers premature exhaustion of hematopoietic stem cells in mice

   

  •  we developed new spheroid-plug model to study tumor angiogenesis

  • multiparameter flow cytometry
    and cell sorting
      ​

  • single cell functional analysis

   

  • transgenic and humanized mice models

   

  • low input RNA-seq

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