HEMATOPOIETIC STEM CELLS
Our body produces millions of new blood cells each second. All of these blood cells derive from hematopoietic stem cells (HSCs). For proper functioning, HSC requires the specialized microenvironment – the niche. However, during aging the blood forming potential of this dynamic system declines. Additionally, HSCs accumulate mutations what may lead to leukemia.
We study how HSCs contribute to blood production during aging and their role in hematopoietic malignancies. Our research focuses on HSCs' heterogeneity and mechanisms that regulate their aging. We trace the roots of leukemia within the pool of human HSCs. Finally, we want to find the cellular and molecular mechanism that governs the specific HSC-niche interactions.
To answer these question we develop new mice models and cooperate with the leaders in the field.
>>> TEAM MEMBERS
>>> CURRENT PROJECTS
Role of Neogenin-1/Netrin-1 axis
in aging of hematopoietic stem cells
Contribution of pre-leukemic hematopoietic stem cells
to acute lymphoid and myeloid leukemias
Heterogeneity and function
of bone-marrow endothelial cells
Finding the determinants of human hematopoietic and leukemic stem cells niche.
>>> OUR ACHIEVEMENTS
>>> OUR METHODS
in cooperation with Irv Weissman laboratory, we demonstrated that Neogenin-1 marks the myeloid-biased fraction of strictly defined Hoxb5+ HSC in mice
we showed that lack of extrinsic
heme-oxygenase-1 triggers premature exhaustion of hematopoietic stem cells in mice
we developed new spheroid-plug model to study tumor angiogenesis
multiparameter flow cytometry
and cell sorting
single cell functional analysis
transgenic and humanized mice models
low input RNA-seq