BEC paper published in Nature Communications
New paper from the Butcher Lab was published in Nature Communications. Agata Szade is one of the co-first authors. The work presents a molecular map of blood vessels in the lymph nodes and defines the subpopulations of endothelial cells involved in leukocyte recruitment.
The development of the inflammatory process is regulated by specialized endothelial cells that control the recruitment of leukocytes from the blood into the lymphoid tissues. The course and physiological effects of the interaction of leukocytes with vascular wall cells have been studied for years and relatively well understood. In contrast, the diversity of blood endothelial cells (BECs) and their origin during inflammatory angiogenesis were not fully characterized so far.
The study uses scRNA-seq to define and characterize different subsets of blood endothelial cells in the mouse lymph node. This includes a previously unknown capillary resident precursor (CRP) and “transitional” endothelial cells (TrEC) that express both capillary and high endothelial cell markers
Understanding the mechanisms that regulate angiogenesis in lymphoid tissues could lead to new strategies for the specific and precise regulation of inflammation.
The study was done during Agata’s postdoc training in Eugene Butcher’s laboratory in Stanford.
The full version of the article is available at the journal website:
K. Brulois*, A. Rajaraman*, A. Szade*, S. Nordling*, A. Bogoslowski, D. Dermadi, M. Rahman, H. Kiefel, E. O’Hara, JJ. Koning, H. Kawashima, B. Zhou, D. Vestweber, K. Red-Horse, RE. Mebius, RH. Adams, P. Kubes, J. Pan, EC. Butcher: A molecular map of murine lymph node blood vascular endothelium at single cell resolution Nat Commun 2020 Jul 30;11:3798. doi: 10.1038/s41467-020-17291-5