G-quadruplexes! Hmox1 longs to be close to you...
New Year brought us new grant and a new publication. In our very recent paper, we show that heme oxygenase-1 (Hmox1) influences G-quadruplexes (G4) accumulation, but rather as one of the proteins regulating the heme availability, not as a rate-limiting factor. Cellular G4–protein colocalizations can be quantitatively analyzed using proximity ligation assay (PLA), even in rare cells. Using PLA we showed that not only Brip1, but also Hmox1, long to to be close to G4 in living cells.
Krzeptowski W, Chudy P, Sokołowski G, Żukowska M, Kusienicka A, Seretny A, Kalita A, Czmoczek A, Gubała J, Baran S, Klóska D, Jeż M, Stępniewski J, Szade K, Szade A, Grochot-Przęczek A, Józkowicz A, Nowak WN. Proximity Ligation Assay Detection of Protein–DNA Interactions—Is There a Link between Heme Oxygenase-1 and G-quadruplexes? Antioxidants. 2021; 10(1):94.
You can find the paper here: https://www.mdpi.com/2076-3921/10/1/94